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axio observer z1 inverted tirf microscope  (Carl Zeiss)


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    Carl Zeiss axio observer z1 inverted tirf microscope
    Axio Observer Z1 Inverted Tirf Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 2970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axio observer z1 inverted tirf microscope/product/Carl Zeiss
    Average 99 stars, based on 2970 article reviews
    axio observer z1 inverted tirf microscope - by Bioz Stars, 2026-03
    99/100 stars

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    Ion effect on microtubule damage. a) Scheme of the damage assay. Salts were added during the damage step. b) Representative <t>TIRF</t> images of microtubules showing progressive and bent damage over time. The first two columns show the same microtubules. Red arrowhead: damage site; yellow arrowhead: breakage of microtubule followed by depolymerization. Scale bar: 5 μm. c) Mean microtubule breaking (number of broken microtubules/the total number of microtubules). d) Mean time until microtubule breakage (with the indicated salt), from the beginning of the assay until microtubule started to depolymerize, as indicated by the yellow arrowhead in (b). e) Mean time until microtubule damage (with the indicated salt), from the beginning of the assay until the first sign of damage. f) Mean lifetime of damaged microtubules (seconds until microtubule depolymerized—seconds of first damage sign), with the indicated salt. d–f) Experimental mean with SD, n = at least three independent experiments, individual values, and experimental mean values (orange dots). Statistics: one-way ANOVA. g) Percentage between the two different damaged types with indicated 100 mM salt or 0 mM (control). h) Scheme of the ionic effects of Na + and Ac − on the longitudinal and the lateral interactions of tubulin within the microtubule structure.
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    https://www.bioz.com/result/axio observer inverted tirf microscope 3i/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    axio observer inverted tirf microscope 3i - by Bioz Stars, 2026-03
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    axio observer inverted tirf microscope  (Carl Zeiss)
    99
    Carl Zeiss axio observer inverted tirf microscope
    Ion effect on microtubule damage. a) Scheme of the damage assay. Salts were added during the damage step. b) Representative <t>TIRF</t> images of microtubules showing progressive and bent damage over time. The first two columns show the same microtubules. Red arrowhead: damage site; yellow arrowhead: breakage of microtubule followed by depolymerization. Scale bar: 5 μm. c) Mean microtubule breaking (number of broken microtubules/the total number of microtubules). d) Mean time until microtubule breakage (with the indicated salt), from the beginning of the assay until microtubule started to depolymerize, as indicated by the yellow arrowhead in (b). e) Mean time until microtubule damage (with the indicated salt), from the beginning of the assay until the first sign of damage. f) Mean lifetime of damaged microtubules (seconds until microtubule depolymerized—seconds of first damage sign), with the indicated salt. d–f) Experimental mean with SD, n = at least three independent experiments, individual values, and experimental mean values (orange dots). Statistics: one-way ANOVA. g) Percentage between the two different damaged types with indicated 100 mM salt or 0 mM (control). h) Scheme of the ionic effects of Na + and Ac − on the longitudinal and the lateral interactions of tubulin within the microtubule structure.
    Axio Observer Inverted Tirf Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axio observer inverted tirf microscope/product/Carl Zeiss
    Average 99 stars, based on 1 article reviews
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    Ion effect on microtubule damage. a) Scheme of the damage assay. Salts were added during the damage step. b) Representative TIRF images of microtubules showing progressive and bent damage over time. The first two columns show the same microtubules. Red arrowhead: damage site; yellow arrowhead: breakage of microtubule followed by depolymerization. Scale bar: 5 μm. c) Mean microtubule breaking (number of broken microtubules/the total number of microtubules). d) Mean time until microtubule breakage (with the indicated salt), from the beginning of the assay until microtubule started to depolymerize, as indicated by the yellow arrowhead in (b). e) Mean time until microtubule damage (with the indicated salt), from the beginning of the assay until the first sign of damage. f) Mean lifetime of damaged microtubules (seconds until microtubule depolymerized—seconds of first damage sign), with the indicated salt. d–f) Experimental mean with SD, n = at least three independent experiments, individual values, and experimental mean values (orange dots). Statistics: one-way ANOVA. g) Percentage between the two different damaged types with indicated 100 mM salt or 0 mM (control). h) Scheme of the ionic effects of Na + and Ac − on the longitudinal and the lateral interactions of tubulin within the microtubule structure.

    Journal: PNAS Nexus

    Article Title: Modulation of microtubule dynamics by monovalent ions

    doi: 10.1093/pnasnexus/pgae544

    Figure Lengend Snippet: Ion effect on microtubule damage. a) Scheme of the damage assay. Salts were added during the damage step. b) Representative TIRF images of microtubules showing progressive and bent damage over time. The first two columns show the same microtubules. Red arrowhead: damage site; yellow arrowhead: breakage of microtubule followed by depolymerization. Scale bar: 5 μm. c) Mean microtubule breaking (number of broken microtubules/the total number of microtubules). d) Mean time until microtubule breakage (with the indicated salt), from the beginning of the assay until microtubule started to depolymerize, as indicated by the yellow arrowhead in (b). e) Mean time until microtubule damage (with the indicated salt), from the beginning of the assay until the first sign of damage. f) Mean lifetime of damaged microtubules (seconds until microtubule depolymerized—seconds of first damage sign), with the indicated salt. d–f) Experimental mean with SD, n = at least three independent experiments, individual values, and experimental mean values (orange dots). Statistics: one-way ANOVA. g) Percentage between the two different damaged types with indicated 100 mM salt or 0 mM (control). h) Scheme of the ionic effects of Na + and Ac − on the longitudinal and the lateral interactions of tubulin within the microtubule structure.

    Article Snippet: Microscopy images were taken with an Axio Observer Inverted TIRF microscope (Zeiss, 3i) and a Prime BSI (Photometrics).

    Techniques: Control